Analgesic Activity of Thyme (Thymus vulgaris L.) Leaf Extract Using Chemical Stimulus Method in Male White Mice (Mus musculus)

Abstract

Pain is an uncomfortable feeling that can also be felt by every individual who has a subjective nature, which can affect the mind and can change life, pain or pain in a part of the body is a problematic pain in one part of the body, Pain can be overcome by the use of analgesic drugs, which can reduce or relieve pain without losing consciousness. Analgesic drugs have a working mechanism that is by affecting the threshold of awareness of the feeling of pain. Analgesic drug groups are divided into 2, namely opioid and non-opioid analgesics. The design of this study is Post test Only Control group Design. This study was conducted using 24 mice as test animals divided into 4 groups. K1 was given CMC-Na 0.5%, K2 Na-Diclofenac 13 mg, K3 thyme leaf extract dose 25 mg, K4 was given thyme leaf extract 50 mg, Mice were induced by 1% acetic acid intraperitoneally . Mice were treated orally, then the number of wriggles was counted every 5 minutes for 30 minutes and the % analgesic power was calculated. The data obtained were analyzed by One Way Anova and Post Hoc test . The number of wriggles was 151.67, the percentage of analgesic power was 25.28%, the results of statistical tests showed that each treatment group had an average number of mice wriggles within 1 hour that did not differ significantly (p = 0.654). From the results of the percentage of protection power of mice wriggling for 1 hour, it showed that a dose of 25 mg had a percentage analgesic power of 25.28%, which was the optimum dose that had the best analgesic ability. Thyme leaf extract (Thymus vulgaris L. ) had analgesic activity in white male mice induced by 1% acetic acid. The optimum dose of 25 mg had the highest analgesic power of 25.28%.