Optimization of Solvent Variations from Fruit Skin Extracts Breadfruit Artocarpus Altilis (Parkinsom Ex F.A,Zom) Fosberg on Analgetic Activity In Vivo

Abstract

Breadfruit peel has analgesic properties because it is known to contain flavonoids that can inhibit cyclooxygenase enzymes. This study aims to determine the analgesic activity of 70% ethanol and methanol extracts from the skin of the breadfruit Artocarpus altilis (Parkinsom ex F.A.Zom) Fosberg in vivo. The method used in this study was the Sigmund method, in which acetic acid was used as an inducer. 40 white male mice aged 2-3 months, weighing 20–30 grams, were divided into 8 groups, namely negative control (CMC-Na 0.5%), positive control (antalgic), groups of 70% ethanol and methanol extract doses of 100 mg/KgBW, doses of 300 mg/KgBW, and doses of 600 mg/KgBW. The parameters observed were the number of mice stretching every 10 minutes for 1 hour. Stretching data is calculated by percent protection and percent effectiveness. Furthermore, statistical analysis was carried out with SPSS 22, and data were analyzed with one-way ANOVA and post hoc LSD to see significant differences between treatments. The results of the one-way ANOVA test showed that there were significant differences in all groups, with a significant value of 0.05. The results of the posthoc test analysis showed no significant difference between the positive control with 70% ethanol extract and methanol at a dose of 600 mg/KgBB (P > 0.05). 70% ethanol and methanol extracts can provide analgesic potency, and the optimal dose, which has nearly the same activity as the positive control, is a dose of 600 mg/kg BW.