Effectiveness Ethanol Extract of Gynura segetum (Lour.) Merr. Leaves on Incision Wound Healing in Male White Mice (Mus musculus)
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Abstract
A wound is damage to tissue continuity caused by injury or surgery. Wounds can be classified into acute and chronic wounds, one type of acute wound being an incision wound, which results from a cut made by a sharp object. One medicinal plant that can be used for treatment is daun dewa (Gynura segatum [Lour.] Merr.), which has properties that are highly beneficial and supportive in the care of clean wounds. It contains flavonoid compounds that act as antimicrobial agents, saponins that act as anti-inflammatory, antibacterial, and antifungal agents useful in the wound-healing process, and essential oil that acts as an antibacterial agent. This study aimed to determine the effectiveness of 96% ethanol extract of daun dewa on the healing of incision wounds in male white mice (Mus musculus), using an experimental pretest-posttest design. Extraction was performed by maceration with 96% ethanol. Male white mice were randomly divided into five groups: a negative control, a positive control, and treatment groups 1, 2, and 3. The mice were anesthetized with 0.1 mL of ketamine intramuscularly; an incision 2 cm long and 2 mm deep was then made on the shaved and cleaned back of each mouse. The wounds received a positive control of povidone iodine, or extract concentrations of 25%, 50%, and 75%, and were observed for 14 days. Phytochemical screening of daun dewa showed positive results for flavonoids, saponins, and essential oil. On day 14, healing percentages were 93% for K(+), 92% for P3, 83% for P2, and 71% for both P1 and K(-). The effective concentration of 96% ethanol extract of daun dewa for healing incision wounds in male white mice was found to be 50%.
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