Effectiveness Of A Combination Of Lady's Tea Extract (Aloe Vera L.) And Celedri Leaf Extract (Apium Graveolens L.) In Healing Wounds In Rats (Rattus Norvegicus)
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Abstract
Abstract: Incision wounds are tissue injuries caused by sharp objects that require rapid and effective healing. Aloe vera and celery leaves are known to contain secondary metabolites such as flavonoids, tannins, and saponins, which are believed to accelerate wound healing. This study aims to determine the effectiveness of combining both extracts in promoting the healing of incision wounds in male rats and to identify their secondary metabolite content. This experimental study used a Post-test Only Control Group Design. Male rats were divided into five groups: negative control (lanolin ointment), positive control (gentamicin ointment), and three treatment groups receiving combinations of aloe vera and celery extracts at ratios of 24.8%:72.5%, 48.6%:48.6%, and 72.5%:24.8%. A 2 cm incision wound was made on each rat and observed macroscopically on days 3, 6, and 9. Wound healing percentage was calculated based on wound length. Phytochemical screening was conducted to identify the secondary metabolites in both extracts. The combination of aloe vera and celery extracts at a ratio of 48,6%:48,6% showed the highest healing percentage, with 40% on day 3, 85% on day 6, and 89% on day 9. Phytochemical tests confirmed the presence of flavonoids, tannins, and saponins in both extracts, which contribute to the wound healing process. Aloe Vera extract and Celery leaf extract were proven to contain secondary metabolite compounds in the form of flavonoids, tannins, and saponins. The combination of Aloe Vera extract and Celery leaf extract can heal cuts in mice. The optimal concentration can be in a ratio of 48,6%: aloe vera and 48,6% celery leaves.
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Abdullah, S, A Saad, A, A, Bota, M, Adnan, J. 2024 Wound healing effect test of red fruit extract gel preparation (pandanus conoideus lamk) on mice (mus musculus) incision wound, Jurnal Farmasi Pelamonia/Journal Pharmacy Of Pelamonia, 04(1): 1–6.
Afriani, N., Idiawati, N. & Alimudidin, A.H. 2016. Phytochemical screening and toxicity test of mentawa root extract (Artocarpus anisophyllus) against artemia salina larvae, Jurnal Kimia Khatulistiwa, 5(1): 58–64.
Algifary, D.F. 2021. Effect of aloe vera extract on wound closure in mice swiss webster. Skripsi . Bandar Lampung: Fakultas kedokteran universitas lampung bandar lampung.
BPOM RI 2019. Persyaratan keamanan dan mutu obat tradisional, Bpom Ri, (32): 1–37.
Fahrezi, R. & Sumarmin, R. 2021. Test of torch ginger (etlingera elatior) leaf extract against wound healing in mice (Mus musculus), Prosiding Semnas Bio 2021 : 1748–1752.
Gupta, A, Rawat, S. 2017. Clinical importance of aloe vera: Review, Research Journal of Topical and Cosmetic Sciences, 8(1): 30–39.
Gusnadi, D, Taufiq, R, Baharta, E. 2021. Oranoleptic and acceptability tests on cassava tapai-based mousse products as umkm commodities in bandung regency, Jurnal Inovasi Penelitian, 1(12): 1–6.
Hedayati, N., Naeini, M, B., Mohammadinejad, A. & Mohajeri, S, A. 2019. Beneficial effects of celery (Apium graveolens) on metabolic syndrome: A review of the existing evidences, Phytotherapy Research, 33(12).
Hendler, K.G., Canever, J.B., de Souza, L.G., das Neves, L.M.S., de Cássia, R.F.M., Kuriki, H., & Marcolino, A.M. 2021. Comparison of photobiomodulation in the treatment of skin injury with an open wound in mice. Lasers in Medical Science 36(9):1845-1854. https://doi.org/10.1007/s10103-020-03216-7.
Hutabarat, H, J, Simaremare, A, P, R, Sipayung, A, P. 2023. Effectiveness of aloe vera extract cream (Aloe vera Linn) on the healing process of incision wounds in rabbit skin (Oryctolagus cuniculus), Magazine of medicine andalas, 46(2):436–443.
Ingallina, C., Capitani, D., Mannina, L., Carradori, S., Locatelli, M., Di Sotto, A., Di Giacomo, S., Toniolo, C., Pasqua, G., Valletta, A., Simonetti, G., Parroni, A., Beccaccioli, M., Vinci, G., Rapa, M., Giusti, A.M., Fraschetti, C., Filippi, A., Maccelli, A., Crestoni, M.E., Fornarini, S. & Sobolev, A.P. 2020. Phytochemical and biological characterization of Italian “sedano bianco di Sperlonga” protected geographical Indication celery ecotype: A multimethodological approach, Food Chemistry.
Kemenkes RI 2017. Farmakope Herbal Indonesia. Edisi II, Kementerian Kesehatan Republik Indonesia. Edisi II.Jakarta: Kementerian Kesehatan Republik Indonesia.
Melanie, R., Kosaric, N., Bonham, Cl, A.& & Gurtner, G, C. 2019. Wound healing: a cellular perspective, Physiological Reviews, 99,: 665–706.
Muliyah, Dyah Aminatun, Sukma Septian Nasution, Tommy Hastomo, Setiana Sri Wahyuni Sitepu, T. 2020. Macam-macam luka, Journal GEEJ, 7(2).
Lau, L., Ni, N., Dayal, H., Lim, S., Ren, Y. & Li, S, F, Y. 2021. Evaluation of anti-inflammatory effects of celery leaf and stem extracts in lps-induced raw 264.7 cells using nitric oxide assay and lc-ms based metabolomics, Current Issues in Molecular Biology, (43): 1876–1888.
Nazar, A. 2023. Antibacterial activity test of ethanol extract of celery herb (Apium graviolens l) against bacteria staphylococcus aureus atcc 25923 Dengan Metode Difusi, Jurnal Kesehatan, 10(1).
Nolff, M.C., Albert, R., Reese, S., & Meyer Lindenberg, A. 2018. Comparison of negative pressure wound therapy and silver-coated foam dressings in open wound treatment in dogs: a prospective controlled clinical trial. Veterinary and comparative orthopaedics and traumatology 31(04):229-238. https://doi.org/10.1055/s-0038-1639579.
Novitasari, A.E. & Putri, D.Z. 2016. Isolation and identification of saponins in mahkota dewa leaf extract by maceration extraction, Jurnal Sains, 6(12):10–14.
Priamsari, M.R. & Yuniawati, N.A. 2019. Phytochemical screening and burn wound healing activity of ethanolic extract of morinda citrifolia l. on rabbit skin. (Oryctolagus Cuniculus), 8(1): 22–28.
Primadina, N., Basori, A. & Perdanakusuma, D.S. 2019. The wound healing process is viewed in terms of cellular and molecular mechanisms, Qanun Medika -Medical Journal Faculty of Medicine Muhammadiyah Surabaya.
Qomariah, S., Lisdiana & Christijanti. Wulan 2015. Effectiveness of fracture stem extract ointment (euphorbia tirucalli) on white rat incision wound healing (rattus novergicus), Unnes Journal of Life Science, 3(2): 79–86.
Riset Kesehatann Dasar (RISKESDAS).2018.Laporan nasional RISKESDAS, Laporan Riskesdas Nasional 2018. Jakarta: Badan Penelitian dan Pengembangan Kesehatan, Kemennterian Kesehatan Repubik Indonesia.
Safriana, Andilala, Fatimah, C. & Samran 2021. Phytochemical profi l of simplisia and ethanol extract of kedondong pagar (lannea coromandelica (houtt) merr .), Jurnal Ilmu Kefarmasian Indonesia, 19(2): 226–230.
Sapri, Eka, S. & Yulianti, A. 2017. Anti-inflammatory activity test of aqueous fraction of celery leaf extract (Apium graveolens l.) in male mice, Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan, 2(Vol 2, No 1 (2017)): 60–67.
Sulistyarini, I., Sari, D, S., & & Wicaksono, T, A. 2016. Phytochemical screening of dragon fruit stem secondary metabolite compounds phytochemical screening of dragon fruit stem secondary metabolite compounds(Hylocereus polyrhizus), Jurnal Ilmiah Cendekia Eksakta, : 56–62.
Syahadat, A. & Siregar, N. 2020. Phytochemical screening of katuk leaves (Sauropus androgynus) as a breast milk facilitator, Jurnal Kesehatan Ilmiah Indonesia, 5(1): 85–89.