In Vitro Acute Toxicity With LC50 Value and Phytochemical Identification of Jamu Lancar Haid T® Using Brine Shrimp Lethality Test (BSLT) Method

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Published: Jul 31, 2025
Keywords:
BSLT, Phytochemicals, T® menstrual flow herbal medicine, LC50, Toxicity Test
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Hasty Martha Wijaya (*)
Institut Teknologi Kesehatan Cendekia Utama Kudus
martha.cendekia@gmail.com
Dian Arsanti Palupi
Institut Teknologi Kesehatan Cendekia Utama Kudus
Salma Zumalia
Institut Teknologi Kesehatan Cendekia Utama Kudus

(*) Corresponding Author

Abstract

Toxicity is the ability of a substance to cause toxic effects (poison) at a certain time. The purpose of this study was to determine the secondary metabolites contained in T® smooth menstruation herbal medicine and the LC50 value in Artemia Salina Leach shrimp larvae. T® smooth menstruation herbal medicine is a herbal product commonly used by women to facilitate menstruation, but has the potential for misuse. The study used a true experimental design with a post-test only control group design. Phytochemical screening showed that the sample contained alkaloids, flavonoids, saponins, and tannins. The toxicity test was carried out using the Brine Shrimp Lethality Test (BSLT) method at five concentration variations (0, 250, 500, 1000, and 2000 ppm), each with three repetitions. The results of data analysis using linear regression y = 1.1643x + 1.5472 showed an LC50 value of 932.017 ppm. This value indicates that the T® herbal medicine for smooth menstruation is toxic to Artemia salina larvae.

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How to Cite
Wijaya, H. M., Palupi, D. A., & Zumalia, S. (2025). In Vitro Acute Toxicity With LC50 Value and Phytochemical Identification of Jamu Lancar Haid T® Using Brine Shrimp Lethality Test (BSLT) Method. Proceeding Cendekia International Conference Health and Technology, 3, 378–385. Retrieved from https://proceedings.centamaku.ac.id/article/view/230
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Vol. 3 (2025): Palliative Care: A Holistic Approach to Addressing the Needs of Patients with Life-Limiting Illnesses
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