Analgesic Activity Test of the Ethanol Extract Combination of Kersen Leaves (Muntingia calabura L.) and Lamtoro Leaves (Leucaena leucocephala (Lam) de Wit) on Acetic Acid-Induced Mice
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Analgesics are drugs that selectively reduce pain that act in the central nervous system or suppress the sensitivity of pain receptors to mechanical stimuli. The presence of dangerous side effects caused by chemical drugs, the use of natural ingredients as drugs tends to increase. Kersen leaves (Muntingia calabura L.) and leaf lamtoro (Leucaena leucocephala (Lam) de Wit) have the same content, namely flavonoids which have analgesic activity. Flavonoids can function as analgesics because they can inhibit the formation of prostaglandin as a mediator of pain sensations. Flavonoids act as analgesics whose mechanism of action can inhibit the work of the enzyme cyclooxygenase which catalyzes the change of arachidonic acid into prostaglandins so as to reduce pain (Ishak, 2017). The purpose of this study was to determine the analgesic activity in kersen leaf extract, lamtoro leaves and a combination of both. This study is an experimental study using stretching method. The test animals used were male mice (Mus Musculus) types of Swiss mice. This study used 40 mice and divided into 8 groups: healthy mice group, negative control positive control, dose group ethanol extract of kersen leaf 50 gram / BB, dose of ethanol extract leaf lamtoro 50 gram / BB, dose combination ethanol extract kersen leaves and lamtoro (1: 2, 1: 1, 2: 1). Data were obtained from observing the amount of stretching of mice by giving each test solution as much as 1mL orally the ethanol extract stock solution of kersen leaves and lamtoro leaves. After 30 minutes all groups of animals treated were intraperitonially injected with 3% v / v acetic acid solution. The data were then analyzed statistically using the SPSS application using the One Way ANOVA method. The results showed that the ethanol extract of leaves of kersen dose of 50 grams / BB, leaves of lamtoro dose of 50 grams / BB, dose combination of etano extract of kersen leaves and leaves of lamtoro 1: 2, 1: 1, 2: 1 had analgesic activity. The combination dose of ethanol extract of kersen leaves and lamtoro leaves is effective as an analgesic, namely a combination dose of 2: 1.
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